Figure 3

Cdc7 silencing or CKIε overexpression restores chromosome decatenation in geminin-silenced cells and leads to cell death. (A) Cdc7 silencing in control-silenced (1, 2), geminin (3, 4) or TopoIIα-silenced (5, 6) H2B-GFP HME cells, or CKIε overexpression in control (7, 8), geminin (9, 10) or TopoIIα (11, 12) H2B-GFP HME cells. (B) Shown are the percentages of cells with anaphase or telophase chromosomal bridges in cells that were control-silenced (n = 297, white bars), Cdc7-silenced (n = 284, yellow bars), geminin-silenced (n = 305, pink bars), geminin/Cdc7-silenced (n = 310, light blue bars), geminin-silenced/CKIε-overexpressing (n = 291, medium blue), TopoIIα-silenced (n = 301, orange bars), TopoIIα/Cdc7-silenced (n = 304, dark blue bars), TopoIIα-silenced/CKIε-overexpressing (n = 277, red bars), geminin/TopoIIα-silenced (n = 301, black bars). Values presented are means ± SD. **P ≤ 0.01. (C) Shown are percentages of viable cells (white bars) or apoptotic cells (with high caspase activity; black bars) following control, Cdc7, geminin, geminin/Cdc7, TopoIIα, TopoIIα/Cdc7 and geminin/TopoIIα silencing, as well as geminin silencing/CKIε overexpression and TopoIIα silencing/CKIε overexpression, as well as geminin/TopoIIα silencing. Values presented are means ± SD. **P ≤ 0.01.