Table 1 Antibodies, epitopes, sources, and dilutions
From: Optimal tumor sampling for immunostaining of biomarkers in breast carcinoma
Protein | Species | Clone | Dilutions | Supplier |
|---|---|---|---|---|
ER | Mouse mAb | 1D5 | 1:50 | Dako |
HER-2 | Rabbit pAb | A0485 | 1:2,000 | Dako |
AKT | Rabbit mAb | 11E7 | 1:1,000 | CST |
ERK1/2 | Mouse mAb | L34F12 | 1:1,000 | CST |
S6K1 | Rabbit mAb | 49D7 | 1:450 | CST |
GAPDH | Rabbit mAb | 14C10 | 1:500 | CST |
Cytokeratin | Rabbit pAb | Z0622 | 1:100 | Dako |