Figure 5

Evidence of ER-AGR2 crosstalk. (a) ZR-75-1 cells were treated with vehicle control (DMSO) or E2 (10 nM) for 24 hours analyzed with Western blot. Numbers above bands represent relative AGR2 induction with E2 treatment after quantitation and normalized to β-actin (Image J). (b) Lysates 72 hours after AGR2 knockdown were analyzed with Western blot for ER. **P < 0.01. Note: Some blots may be from different gels run with the same set of samples.