Figure 1From: The membrane mucin MUC4 is elevated in breast tumor lymph node metastases relative to matched primary tumors and confers aggressive properties to breast cancer cellsCharacterization of 1G8 antibodies. (a) Whole cell lysates from the indicated breast epithelial cell lines were resolved by 8% SDS-PAGE and blotted with commercially obtained anti-MUC4 antibody 1G8 (1G8c). MCF7-Rep5 is a human breast cancer line stably expressing inducible rat MUC4 (24). (b) Cell lysates were resolved by 6% to 12% gradient SDS-PAGE and immunoblotted with supernatant from cultured 1G8 hybridoma (1G8s) or with anti-tubulin. The MATB-III rat mammary adenocarcinoma cell line was used as a positive control for MUC4 expression (12, 44, 45). (c) Wild-type JIMT-1 or JIMT-1 cells stably transduced with MUC4 shRNA were blotted with 1G8s and actin. (d) HEK293T cells were transiently co-transfected with pLenti vector control or pLenti-hMUC4/Y vector, together with pSuper vector control (v) or MUC4 shRNA (M4) or scrambled (scr) oligonucleotides in pSuper. Lysates were blotted with 1G8s and actin.Back to article page