Figure 2

Mutation of a YY1 site in an HP1α gene promoter reporter construct has a more pronounced effect in MCF7 cells than in HS578T cells. (a) Reporter constructs representing the HP1α promoter region. Three HP1α constructs with the same 3' end and different truncations of the 5' end were transfected into MCF7 and HS578T cells. The luciferase activity of each construct is represented as a percentage of the activity of the CbxD98 construct. RLU = relative luciferase units. (b) Mutational analysis of the CbxU283 construct. The luciferase activity of each construct is represented as a percentage of the activity of the wild type construct. Only mutation of site ying yang 1 (YY1).2 consistently resulted in a differential effect in MCF7 and HS578T cells. P value was calculated using a two-sample unequal variance student t-test. * P = 0.002.