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Table 2 Detection of CK19 in cancer cell lines by different methods

From: Full-length cytokeratin-19 is released by human tumor cells: a potential role in metastatic progression of breast cancer

 

Colorectal cells

Breast cells

Head/Neck cells

Thyroid cells

 

HT29

HCT116

Caco-2

MCF7

14C

22A

ML1

C643

Detection of intracellular CK19

   FC

+

+

+

+

-

-

-

-

   ICC

+

+

+

+

-

-

-

-

   WB

ND

ND

ND

+

-

-

ND

-

Detection of released CK19

   EPISPOT

+

+

+

+

-

-

-

-

   ELISA

+

+

+

+

-

-

-

-

  1. CK19 detection. FC = flow cytometry; ICC = immunocytochemistry; WB = Western blot; ND = not determined; EPISPOT = EPithelial ImmunoSPOT assay; ELISA = CYFRA 21-1 immunometric assay (B.R.A.H.M.S.).