Figure 1From: Genes responsive to both oxidant stress and loss of estrogen receptor function identify a poor prognosis group of estrogen receptor positive primary breast cancersExperimental conditions and derivation of gene signatures based on MCF7 treatments and microarray analyses. Depicted by the left set of conditions, the Ox' gene signature (color-coded in gold) was derived as the overlapping probe set after significance analysis of microarrays (SAM) comparisons of expression microarrays results from MCF7 treated with both estrogen receptor (ER)-α small-interfering RNA (siRNA) and oxidants, relative to MCF7 treated only with ER-α siRNA. Depicted by the middle set of conditions, the estrogen (E)/ER gene signature (color-coded in blue) was derived as the overlapping set of differentially expressed MCF7 probes following estradiol (E2) deprivation and ER-α knockdown. Depicted by the right set of conditions, the Ox signature (color-coded yellow) was derived as the overlapping probe set after SAM comparisons of microarray results from oxidant stressed MCF7 relative to control MCF7. The overlap between the Ox' and E/ER signatures defines the Ox'-E/ER signature (color-coded dark green); and the overlap between the Ox and E/ER signatures defines the Ox-E/ER gene signature (color-coded light green).Back to article page