Fig. 5
Changes in vasculature of 67NR tumors after ICI treatment. a Exemplary Ktrans and T1 maps post-gadofosveset injection of ICI-treated 67NR tumors compared to untreated controls. b Area under the curve (AUC), maximum slope (slopemax), Ktrans and ∆T1 analyses indicating decreasing tumor perfusion and permeability parameters on day six and day nine. Each dot represents one animal, with horizontal lines indicating the mean. c H&E stainings of 67NR tumor sections show areas of intratumoral hemorrhage after treatment, which was not visible in control tumors. CD31 expression is reduced. Scale bars represent 50 µm. d Transmission electron micrographs (TEM) of 67NR tumors. Untreated 67NR tumors show a continuous endothelial layer with intact basal lamina. Also ICI-treated tumors show intact endothelial layers with apparently thicker endothelium. Scale bars represent 1 µm. e erythrocyte, el endothelial layer, bl basal lamina, cf collagen fibrils. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001