Fig. 3
Changes in vasculature of 67NR tumors after sorafenib treatment. a Exemplary Ktrans and T1 maps post-gadofosveset injection of sorafenib-treated 67NR tumors compared to untreated controls. b Area under the curve (AUC), maximum slope (slopemax), Ktrans and ∆T1 analyses indicating decreasing tumor perfusion and permeability parameters on day six and day nine. Each dot represents one animal, with horizontal lines indicating the mean. c H&E stainings of 67NR tumor sections show increasing areas of necrosis after treatment, while CD31 expression, especially on day nine, is reduced. Scale bars represent 50 µm. d Transmission electron micrographs (TEM) of 67NR tumors. Untreated 67NR tumors show a continuous endothelial layer with intact basal lamina. Sorafenib-treated tumors show similar characteristics with overall thicker endothelium. Scale bars represent 1 µm. n nucleus, e erythrocyte, el endothelial layer, bl basal lamina, cf collagen fibrils. **p < 0.01, ****p < 0.0001