Fig. 1

Wnt/PCP signaling mediates breast cancer cell migration. a Relative cell migration quantification of BT549, MDA-MB-231,MDA-MB-468, SkBr3, NDL, MCF7, and T47D cells stably expressing Control, shVangl2-1, or shVangl2-2 (BT549 n = 3, p = 4.81E-05 and p = 4.47E-05, MDA-MB-231 n = 4, p = 0.0001 and p = 2.73E-05, MDA-MB-468 n = 3, p = 0.0078 and p = 0.0146, SkBr3 n = 3, p = 0.003 and p = 0.0057, NDL n = 3, p = 0.0250 and p = 0.0063, MCF7 n = 3, p = 0.0180 and p = 0004, T47D n = 3, p = 2.13E-05 and p = 1.14E-06). b Representative images of migrating NDL cells stimulated with Vector- or Wnt5a- conditioned media at 0 and 12 h. Scale bar = 200 µm. c Relative cell migration quantification of MDA-MB-231, MDA-MB-468, SkBr3, NDL, and MCF7 cells stimulated with Vector- or Wnt5a-conditioned media (MDA-MB-231 n = 4, p = 0.0006, MDA-MB-468 n = 9, p = 2.33E-06, SkBr3 n = 4, p = 0.0004, NDL n = 4, p = 0.0344, MCF7 n = 10, p = 1.54E-08) d MCF7, MDA-MB-231, MDA-MB-468, Met-1, and NDL cells stimulated with Vector- or Wnt5a-conditioned media for 1 h blotted for Dvl2. e MDA-MB-231, SkBr3, T47D, and MCF7 cells stimulated with Vector- or Wnt5a-conditioned media for 1 h blotted for phospho-Vangl2 and Vangl2. f MDA-MB-231 cells stably expressing Control, shVangl2-1, or shVangl2-2 blotted for Dvl2. g Relative cell migration quantification of MDA-MB-231 cells stably expressing Control, shVangl2-1, or shVangl2-2 stimulated with Vector- or Wnt5a- conditioned media (control: vector- vs Wnt5a-conditioned media n = 4, p = 0.0003, Control vs shVangl2-1 + vector-conditioned media n = 4, p = 0.0003, control vs shVangl2-2 + vector-conditioned media n = 4, p = 0.0003, shVangl2-1: vector- vs Wnt5a-conditioned media n = 4, p = 0.7256, shVangl2-2: vector- vs Wnt5a-conditioned media n = 4, p = 0.5804). Bar graphs represent the mean ± sem of experimental replicates (n). Significance was determined by a two-sided unpaired t test with Welch’s correction, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001