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Fig. 3 | Breast Cancer Research

Fig. 3

From: Claudin-4-adhesion signaling drives breast cancer metabolism and progression via liver X receptor β

Fig. 3

CLDN4 activates SFKs in breast cancer cells via the EC2 and Y197. A, B Confocal images of the indicated proteins in T47D:CLDN4–/–, MCF-7:CLDN4–/– and their parental cells. T47D and MCF-7 cells were grown for 24 h in the presence or absence of 1.0 μg/ml C-CPE. Scale bar, 20 μm. C Western blot for the indicated proteins in the revealed breast cancer cells. The protein levels are normalized to the rehybridized β-actin levels, and the relative levels are shown in the histograms. MM231, MDA-MB-231. D The construct of wild-type (WT) and mutant CLDN4 expression vectors. EF-1α, elongation factor-1α; IRES, internal ribosome entry site. E, F Western blot for the indicated proteins in the revealed T47D cells. G Quantitative BrdU assay for the indicated cells. The BrdU/DAPI levels are plotted and shown in the histograms (mean ± SD; n = 5–6)

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