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Fig. 10 | Breast Cancer Research

Fig. 10

From: Carbonic anhydrases reduce the acidity of the tumor microenvironment, promote immune infiltration, decelerate tumor growth, and improve survival in ErbB2/HER2-enriched breast cancer

Fig. 10

Acetazolamide lowers tumor immune cell infiltration and inflammation. A-C Exemplary immunohistochemical images of tumor sections stained for CD3 (T cells, A), CD19 (B cells, B), and F4/80 (macrophages, C). Tumors were from mice treated with acetazolamide (ATZ) or vehicle. Scale bars are 50 µm, all images are at same magnification. Arrows indicate positive cells; in panel C, only few are highlighted due to the high number of positive cells. D Quantification of the relative density of immune cells within tumors (n = 12–15). We quantified cells within the epithelial tumor mass and intraepithelial connective tissue strands. E Expression data for inflammatory cytokines and transcription factors. Following square root transformation, data were compared by repeated-measures two-way ANOVA and the overall effects of ATZ are reported

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Breast Cancer Research

ISSN: 1465-542X

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