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Fig. 1 | Breast Cancer Research

Fig. 1

From: PKC-ζ mediated reduction of the extracellular vesicles-associated TGF-β1 overcomes radiotherapy resistance in breast cancer

Fig. 1

1D11 improves radiotherapy effects on the tumor growth but not life span in murine 4T1 breast cancer model. A TGF-β1 concentration in EVs secreted by NSCLC tumor specimen. Tumor tissues from NSCLC patients were cultured in vitro for 48 h. Culture medium was collected for EV separation and ELISA was performed for analysis of TGF-β1 n = 6. B Schematic diagram of tumor therapy model. Tumor model was established by injecting 5 × 104 4T1 cells subcutaneously into the fourth mammary pad of BALB/c mice. Radiation (10 Gy) was performed 14 days after injection of tumor cells. Mice were treated by anti-TGF-β1 antibody (1D11, 10 mg/kg) intravenously 3 times per week for 3 weeks. C Tumor tissues were collected at indicated day for flow cytometry analysis. Percentage of CD25+Foxp3+ (Treg) in CD4.+cells n = 6. D Tumor volume was recorded n = 15. E Survival curve in groups of different treatment combination n = 10. F Median survival time in groups of different treatment combination n = 10. Experiments was analyzed by 2-way ANOVA. *p < 0.05

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