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Fig. 5 | Breast Cancer Research

Fig. 5

From: The WAVE3/β-catenin oncogenic signaling regulates chemoresistance in triple negative breast cancer

Fig. 5

Loss of WAVE3 expression or phosphorylation in combination with chemotherapy induce β-catenin degradation. A & B Representative Western blots of protein lysates from parental and CIS-R (A) or DOX-R (B) MDA-MB-231 cells that were treated with or without cisplatin (30 µM) for 24 h, and subjected to immunoblotting with antibodies against β-Catenin. β-Actin was used as loading control. CF Representative Western blots of protein lysates from MDA-MB-231 (C & D) or 4T1 cells (E & F) and their derivatives that were treated with or without cisplatin (C & E) or doxorubicin (D & F) for 24 h, and were subjected to immunoblotting with antibodies against β-Catenin. β-Actin was used as loading control. G Representative confocal microscopic images of sections of tumors derived from mice injected with parental (CTRL), cisplatin resistance (CIS-R) or WAVE3-deficient CIS-R (CIS-R-W3KO) MDA-MB-231, and stained for β-catenin (upper panels, green) or WAVE3 (lower panels, green). Cell nuclei were counterstained with DAPI (blue). Scale bar, 100 µm. H and I Representative Western blots of nuclear (N) or cytoplasmic (C) fraction of protein lysates from parental and CIS-R (H) or DOX-R (I) MDA-MB-231 cells that were subjected to immunoblotting with antibodies against β-catenin or WAVE3. J & K Representative Western blots of protein lysates from parental MDA-MB-231 cells and their derivatives that were treated with or without cisplatin or doxorubicin for 24 h, and were subjected to immunoblotting with antibodies against β-catenin. The nuclear fraction is analyzed in (J), while the cytoplasmic fraction is analyzed in (K). α-Tubulin and Lamin B1 were used as loading controls for the nuclear and the cytoplasmic fraction, respectively. Data shown are representative of 3 replicates. The numbers under each WB band represent the fold change in signal intensity with respect to its respective control band in each panel after normalization to the loading control signal

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