Fig. 2

Loss of WAVE3 expression or its phosphorylation in combination with chemotherapeutics inhibits tumorsphere formation and invasion in vitro. A Representative micrographs of tumorspheres derived from parental MDA-MB-231 cell line and its derivatives treated with or without doxorubicin (100 nM) for 14 days. B and C Quantification of the number of tumorspheres formed with or without treatment with cisplatin (500 nM), paclitaxel (100 nM) or doxorubicin (100 nM) of MDA-MB-231 (B) and 4T1 cells (C) and their derivatives. D Representative micrographs of tumorsphere invasion (red arrows point to invasive microspheres) derived from parental MDA-MB-231 cells and its derivatives treated with or without doxorubicin (100 nM) for 10 days. At day 3, Matrigel was added to the tumorsphere cultures and cells that invaded the Matrigel (arrows) were recorded and quantified at day 10. E and F Quantification of the number of invasive microspheres with or without treatment with cisplatin (500 nM), paclitaxel (100 nM) or doxorubicin (100 nM) of MDA-MB-231 (E) and 4T1 (F) and their derivatives. Data are the mean ± SD (n = 3, **p < 0.01, Student’s t test). Data shown is representative of three independent experiments