Fig. 4

Macrophage depletion decreases NF-κB signaling and MenaINV expression in a PDX model in vivo. A Experimental design for macrophage depletion in patient-derived xenograft (PDX) HT17 model in SCID mice. i.p. = intraperitoneal. Red arrows indicate treatment days. B Immunofluorescence co-staining of HT17 tumors xenografted in SCID mice treated as outlined in A for the macrophage marker, Iba1 (white), C p65 (red), MenaINV (green) and nuclei (blue-DAPI). Scale bars = 20 μm. D Quantification of average fold change in p65 expression from mice in A. E Quantification of average fold change in p65 nuclear localization in PDX HT17 tumors from mice treated as outlined in A. Only p65 co-localized with the nuclear DAPI signal was quantified. F Quantification of average fold change MenaINV expression from PDX HT17 tumors treated as outlined in A. Data in D–F were analyzed using a student’s t-test. **p < 0.01, ***p < 0.001. Eight mice were treated per group. Each dot represents the average measurement from an individual mouse