Fig. 3

MenaINV expression in tumor cells induced by macrophages depends partially on TNFα-mediated NF-κB signaling and Notch1 Jagged1 signaling. A MenaINV mRNA expression in MDA-MB-231 (231) cells co-cultured with or without BAC2.1F macrophages (Mac) and with or without C87 (TNFα inhibitor) or SAHM1 (MAML1 inhibitor) for 4 h. B MenaINV mRNA expression in 231 cells co-cultured with or without Macs, TNFα inhibitor (C87), or Jag 1 or Jag2 blocking antibodies for 4 h. Bars in A and B represent average fold change of MenaINV mRNA expression compared to control cells (231). C MenaINV mRNA expression in 231 cells co-cultured with sgControl (WT) or Jagged1 knockout BAC1.2F5 macrophages (Jag1 KO Macs). Bars in (A–C) represent average fold change of MenaINV mRNA expression compared to control cells (A and B: 231; C: 231 + WT Macs). Data were analyzed using a one-way ANOVA with Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s. = not significant. All experiments were repeated at least three times