Fig. 3
From: CMTM7 inhibits breast cancer progression by regulating Wnt/β-catenin signaling
CMTM7 inhibited cell proliferation, migration, and invasion. A The construction of CMTM7-overexpressed MCF-7 cells validated by western blot. The cell proliferation assays were conducted, including MTT (B), colony formation assay (C), and EdU assay (E). The invasion and migration assays were performed, including transwell assay (D), scratch assay (F) and single-cell time-lapse imaging assay (G). H The expression levels of EMT-related biomarkers, including E-cadherin, N-cadherin, and Vimentin in CMTM7-overexpressed MCF-7 cells (left) and CMTM7-depleted MCF-10A cells (right) detected by western blot. The apoptotic cells (I) and the distribution of cell cycle (J) were analyzed through cell cytometry. K The image and growth curve of tumor volume after in situ injection in SCID mice. L The image of bioluminescence and curve after intravascular injection. *p < 0.05; ***p < 0.01