Fig. 5

Blocking E-cad/KLRG1 pathway inhibits tumor metastasis. a qPCR analysis of IFN-γ mRNA expression in tumor-infiltrating CD8+ T cells of MMTV-PyMT mice treated with IgG or Anti-KLRG1 antibody (n = 5, each; left panel). Flow cytometry analysis of tumor-infiltrating GZMB+ CD8+ T cells in MMTV-PyMT mice treated with IgG or Anti-KLRG1 antibody (n = 5, each; right panel). b qPCR analysis of T-bet and IFN-γ mRNA expression in tumor-infiltrating CD4+ T cells of MMTV-PyMT mice treated with IgG or Anti-KLRG1 antibody (n = 5, each). c Flow cytometry analysis of peripheral blood GZMB+ CD8+ T cells and IFN-γ+ CD4+ T cells MMTV-PyMT mice treated with IgG or Anti-KLRG1 antibody (n = 5, each). d Left panel shows the representative images of H&E-stained lung sections from MMTV-PyMT mice treated with IgG or Anti-KLRG1. Scale bar, 2 mm. Right panel shows the quantification of metastatic foci in lungs from MMTV-PyMT mice treated with IgG or Anti-KLRG1 (n = 5, each). Data presented as the mean ± sem and p value was determined by two-tailed t test (a–d)