Fig. 2

Necroptosis of tumor cells promotes T cell activation. a Flow cytometry analysis of tumor-infiltrating CD4+ T and CD8+ T cells in WT (n = 5), Mlkl KO (n = 6) MMTV-PyMT mice at 12 weeks and WT (n = 6), Mlkl KO (n = 6) MMTV-PyMT mice at 15 weeks. No significant difference in p value was observed. b qPCR analysis of IFN-γ mRNA expression in tumor-infiltrating CD8+ T cells of WT and Mlkl KO MMTV-PyMT mice at 12 weeks and WT (n = 6) and 15 weeks (n = 5, each) (left panel). Flow cytometry analysis of tumor-infiltrating GZMB+ CD8+ T cells in WT and Mlkl KO MMTV-PyMT mice at 12 weeks and WT (n = 6) and 15 weeks (n = 5, each) (right panel). c qPCR analysis of T-bet and IFN-γ mRNA expression in tumor-infiltrating CD4+ T cells of WT and Mlkl KO MMTV-PyMT mice at 12 weeks and 15 weeks (n = 6, each). d Flow cytometry analysis of peripheral blood GZMB+ CD8+ T cells and IFN-γ+ CD4+ T cells in WT (n = 5) and Mlkl KO (n = 4) MMTV-PyMT mice at 15 weeks. Data presented as the mean ± sem and p value was determined by two-tailed t test (a–d)