Fig. 2

Progesterone downregulates DSCAM-AS1 via progesterone receptor to suppress invasion and migration of PR-positive breast cancer cells. Real-time PCR analysis of DSCAM-AS1 in response to progesterone and mifepristone + progesterone in A T47-D and B BT-474. DSCAM-AS1 expression is normalized with that of GAPDH, and relative fold change values are plotted in comparison to vehicle control. C Real-time PCR analysis indicating DSCAM-AS1 expression upon PR and GR knockdown followed by progesterone treatment in T47-D cells. Relative fold change is calculated by 2^−ΔΔCT and plotted on Y-axis. p-value calculated using Student’s t-test. Transwell D–E cell migration and F–G invasion assay upon siRNA-mediated silencing of DSCAM-AS1 in T47-D and BT-474 cells. Representative images of crystal violet-stained migrated or invaded cells (10 ×) from each condition are shown. Each bar plot indicates percent cell migration or invasion in each condition with respect to vehicle-treated si-control cells. p-value calculated using Student’s t-test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns non-significant