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Fig. 1 | Breast Cancer Research

Fig. 1

From: Progesterone modulates the DSCAM-AS1/miR-130a/ESR1 axis to suppress cell invasion and migration in breast cancer

Fig. 1

Progesterone deregulates long non-coding RNAs in breast cancer cells. A List of significantly deregulated lncRNAs in the transcriptome sequencing data of breast cancer cell lines and patient samples treated with progesterone. Expression fold change upon progesterone treatment is indicated against each lncRNA. lncRNAs downregulated in primary tumors upon progesterone treatment are highlighted in gray shade. *Represents no expression of the gene in breast primary tumor samples. B–D Real-time PCR analysis of differentially expressed lncRNAs in B T47-D, C BT-474, and D MDA-MB-231 breast cancer cells treated with progesterone. The expression of lncRNAs is normalized with that of GAPDH in the same sample. Changes in the normalized expression of lncRNAs upon treatment are plotted as relative fold change (2−ΔΔCT) with respect to expression in vehicle control for the same cell line. This consists of data from three biological replicates. The horizontal black line represents a normalized expression of lncRNAs in vehicle-treated cells. SGK1, a progesterone-responsive gene, is used as a positive control. p-value calculated using Student’s t-test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns non-significant

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