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Table 1 M87A media recipe. Combine equal volumes of MEBM and DMEM/F12 basal media, then add the supplements as listed in this table. Finally, filter-sterilize with 0.2-Ī¼m polyethersulfone membrane

From: Sustained postconfluent culture of human mammary epithelial cells enrichesĀ for luminal and c-Kit+ subtypes

Factor

Amount

Glutamine

2.0Ā mM

Fetal bovine serum

0.25%

Bovine pituitary extract

35Ā Ī¼g/mL

Insulin

3.0Ā mg/mL

Isoproterenol

5Ā Ī¼M

Hydrocortisone

0.3Ā Ī¼g/mL

Apo-transferrin

2.5Ā Ī¼g/mL

Oxytocin

0.1Ā nM

Cholera toxin

0.5Ā ng/mL

Epidermal growth factor

5.0Ā ng/mL

Ī²-estradiol

500Ā pM

Tri-iodo-thyronine

5Ā nM

Alubmax I

0.1%