Fig. 2

Postconfluent cultures are highly enriched for luminal cells. A Flow cytometry using CD133 to identify LEps and CD271 to identify MEps shows enriched abundance of LEps in postconfluent culture as compared to specimen-matched standard culture. Quantified lineage compositions for each of these flow plots are given in Table 4. n = 5 distinct HMEC specimens were evaluated. B Immunostaining using KRT19 to identify LEps and KRT14 to identify MEps shows enriched abundance of LEps in postconfluent culture as compared to specimen-matched standard culture. C Immunostaining using KRT8 to identify LEps. Scale bars are 100 μm