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Fig. 5 | Breast Cancer Research

Fig. 5

From: Mebendazole prevents distant organ metastases in part by decreasing ITGβ4 expression and cancer stemness

Fig. 5

Mebendazole reduces tumor growth and lung and liver metastases in an immune-competent animal model of metastasis. A Luciferase-labeled 4T1-cells were implanted into the mammary fat pad of Balb/c mice. Tumor growth curves displaying tumor volume over time are plotted for each treatment group (mean ± SEM, N = 10–11 mice). Treatments included a high-fat KetoCal diet (Con; N = 10) or 250 ppm of MBZ incorporated into a high-fat KetoCal diet (Feed; N = 11). P values for two-tailed t test *< 0.05. B Final tumor weights are plotted for mice in each treatment group. P values for two-tailed t test * < 0.05. C, D The bioluminescence intensity (BLI) of each tumor was imaged and quantified on day 12, 19, and 26 in live mice. P values for two-tailed t test *< 0.05. E The BLI of each mouse lung was quantified on the final day of the experiment after resecting (day 26). P values for two-tailed t test * < 0.05. F BLI images of lung metastasis prior to DNA extraction. G Lung metastatic burden was quantified by measuring the DNA content of the luciferase (LUC) gene in each mouse lung divided by individual tumor weights and then normalized to the average value of the control group (mean ± SEM, N = 10–11 mice with n = 3 technical replicates). P values for two-tailed t test ****< 0.0001. H BLI images of liver metastasis prior to DNA extraction. I The total number of mice with liver metastasis based on a bioluminescence threshold of 4000 photons.s−1 cm2 is displayed. Chi-square test **< 0.01

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