Skip to main content
Fig. 3 | Breast Cancer Research

Fig. 3

From: Mebendazole prevents distant organ metastases in part by decreasing ITGβ4 expression and cancer stemness

Fig. 3

Mebendazole reduces the migration of TNBC cells. A, B Quantification of relative wound closure of SUM159 A and MDA-MB-231 B cells treated for 12 h and 24 h with indicated concentrations of MBZ or DMSO as a vehicle control. Corresponding representative photomicrographs are shown at the right A mean ± SEM; N = 3 independent experiments. P values for two-way ANOVA test comparing vehicle control to each individual treatment dose *< 0.05, **< 0.01, ***< 0.001, ****< 0.0001. B Scale bar = .9 mm. C, D MDA-MB-231 and SUM159 cells were pre-treated for 48 h with 0.5 μM or 1 μM of MBZ or DMSO and plated for transwell assays. The number of cells that migrated through the filter was determined by crystal violet staining followed by bright-field imaging (C) and quantified by the percent area of crystal violet staining (D). Mean ± SEM; N = 3 independent experiments. P values for two-way ANOVA test comparing vehicle control to each individual treatment dose **< 0.01,***< 0.001,****< 0.0001

Back to article page