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Fig. 2 | Breast Cancer Research

Fig. 2

From: Ring finger protein 126 promotes breast cancer metastasis and serves as a potential target to improve the therapeutic sensitivity of ATR inhibitors

Fig. 2

RNF126 promotes cell proliferation, cell growth, migration, and invasion of breast cancer cells. A The relative expression levels of RNF126 mRNA in MCF7 (upper panel) and MDA-MB-231 (down panel) cells with or without RNF126 knockdown (Paired t-test). B Cell proliferation assay shows the effects of RNF126 on MCF7 (upper panel) and MDA-MB-231 (down panel) cells at indicated time points (Two-way ANOVA). C Wound healing assay was performed to measure the effect of RNF126 knockdown on the migration of MCF7 (left panel) and MDA-MB-231 (right panel) cells (Paired t-test, scale bar, 200 μm). The migration D and invasion E ability of MCF7 (upper panel) and MDA-MB-231 (down panel) cells with or without RNF126 knockdown was measured by transwell assay (Paired t-test, scale bar, 200 μm). F Tumor growth curves of the mice (n = 5 per group) with or without RNF126 knockdown (MCF7, left panel; MDA-MB-231, right panel). The tumor volume was monitored every 3 days (Two-way ANOVA). G Diagram of the tumor on day 21. H Schematic representation of tumor with or without RNF126 knockdown by IHC. I Weight of tumors derived from mice in each group (MCF7, upper panel; MDA-MB-231, down panel). J Number of metastases of liver and lung in MCF7 (upper panel) and MDA-MB-231 (down panel) with or without RNF126 knockdown in mice (Two-way ANOVA). K Schematic diagram of metastatic nodules. Histological observation of metastatic organs includes of lung, liver visualized using H&E staining. Black arrow: metastasis nodules. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001. All presented results are from three independent experiments

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