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Fig. 6 | Breast Cancer Research

Fig. 6

From: Identification of a novel ER-NFĸB-driven stem-like cell population associated with relapse of ER+ breast tumors

Fig. 6

The MS Cluster 1 signature is associated with more aggressive metastatic disease. A Single-cell transcriptomes from 4OHT-treated MCF-7 cells (GSE181812) and LTED (Long-Term Estrogen Deprived) MCF-7 cells (GSE122743) were integrated and represented bi-dimensionally. B The distribution of 4OHT-treated MCF-7 cells and LTED MCF-7 cells in each cluster relative to the total cell number is shown. C FEA was performed on integrated datasets for the MS Cluster 1 Signature. D Bi-dimensional representation of 6519 single-cell transcriptomes of peripheral blood mononuclear cells derived from patients with metastatic breast cancer are shown (UMAP) with Cluster 13 presenting of Circulation Tumor Cells (CTCs) (GSE174463). E FEA was performed for the custom genes signatures derived from DEGs of each MS Cluster on CTCs Cluster 13. AUC values are shown in a heatmap, and P-values are presented in Additional file 2: Supplemental Table 9. F, I Single-cell transcriptomes from primary and metastatic tumors of PDX models UCD46 (F) and UCD4 (I) are represented in UMAP plots (GSE131007). G, J The proportion of cells in each cluster is indicated by their origin (i.e., primary tumor or metastatic location) relative to the total number of cells of each origin. H, K FEA was performed for the MS Cluster 1 Signature on both datasets with box plots showing signature scores per cluster

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