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Fig. 1 | Breast Cancer Research

Fig. 1

From: Steroid receptor coactivator-3 inhibition generates breast cancer antitumor immune microenvironment

Fig. 1

SI-2 suppresses the growth and lung metastasis of E0771 mammary gland tumors in C57BL/6J mice. A Reduction in luciferase activity in E0771 tumors from C57BL/6J mice by SI-2 (2.5 mg/kg) treatment compared with vehicle treatment. B Quantification of luciferase activity in the E0771 breast tumors shown in Panel A. C Kaplan–Meier plot of mice bearing E0771 tumors treated with SI-2 (2.5 and 10 mg/kg) or vehicle. If the luciferase activity in E0771 tumors in a mouse was greater than 20 × 106 luciferase photons/second/cm2, the mouse was counted as deceased. The Kaplan–Meier plot was generated using GraphPad Prism (version 8.0). D Images and volumes of harvested E0771 tumors treated with SI-2 (2.5 mg/kg) or vehicle. Tumor volume was calculated as 0.5 × length × width × width. The analysis of the tumor volume in mice treated with SI-2 or vehicle is shown in the graph. E H&E staining of E0771 breast tumors treated with SI-2 (2.5 mg/kg) or vehicle. F Images and quantitative analysis of luciferase activity in E0771 cells in the lungs of mice treated with SI-2 (2.5 mg/kg) or vehicle, as shown in Panel A. G H&E staining of lungs harvested from E0771 tumor-bearing mice treated with SI-2 (2.5 mg/kg) or vehicle, as shown in Panel F. Lungs were serially sectioned at 7-µm intervals, and the slices with cancer masses were selected and then stained. Arrowheads indicate tumor masses in the lungs. H Blood cytokine profiles in female mice treated with vehicle or 2.5, 5, or 10 mg/kg SI-2 twice a day for 7 days. The relative fold change of each cytokine of blood from animals treated with SI-2 was calculated compared to the vehicle controls

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