Fig. 4
Gene expression analysis reveals segregation of mammary tumors into patient-defined luminal and basal-like molecular subtypes. A Principal component analysis shows that samples clustered into 3 separate groups, normal mammary glands (blue), B1/P tumors (yellow), and tumors with Rbf inhibition (red, green). B Hierarchical clustering of PAM50 genes divides B1/P tumors from tumors with Rbf inhibition. C Correlation analysis with PAM50 signatures indicates high association of B1/P tumors with the basal-like subtype and tumors with Rbf inhibition with the luminal B subtype. D–E GSEA shows significant enrichment for curated gene sets distinguishing luminal vs basal-like breast cancer. D The gene set upregulated in luminal vs mesenchymal breast cancer is highly expressed in B1/P/Rbf tumors compared to B1/P tumors. E The gene set downregulated in luminal vs basal breast cancer is suppressed in B1/P/Rbf tumors compared to B1/P tumors