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Fig. 6 | Breast Cancer Research

Fig. 6

From: RAB4A GTPase regulates epithelial-to-mesenchymal transition by modulating RAC1 activation

Fig. 6

RAB4A is essential for self-renewal in multiple human cancer cell lines. (A-D) Serial replating sphere formation assay on RAB4A-high cells with and without stable RAB4A knockdown. A, B Representative microscopic images of the replating spheres of PC3 A and SNB19 B cells. C, D Quantification of sphere numbers from A, B analyzed using OpenCFU and Prism software. E–H Serial replating sphere formation assay on RAB4A-low cells with and without multiple forms of RAB4A stable overexpression. E, F Representative microscopic images of the replating spheres of MCF7 E and H1299 F cells. G, H Quantification of sphere numbers from E, F analyzed using OpenCFU and Prism software. Data presented in the bar graphs are mean ± SEM (n ≥ 5). “*” represents p < 0.05 of the comparison made between control and the corresponding group

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