Fig. 5
From: RAB4A GTPase regulates epithelial-to-mesenchymal transition by modulating RAC1 activation
Impact of RAB4A and RAC1 co-manipulation on mesenchymal-epithelial properties and cell invasion in multiple cancer cell lines, A Immunoblot analysis of RAB4A protein expression in human cancer cell lines of diverse tissue origins. B, C Invasion study of the RAB4A high-expressing PC3 B and SNB19 C cells, with and without RAB4A knockdown. Scale bar = 100 µM. D, E qPCR analysis to quantify the expression levels of mesenchymal marker ZEB1 and epithelial marker E-Cadherin (CDH1) upon RAB4A knockdown using the groups of cells as in B, C. The expression levels and changes of these markers are compared to those in the control siRNA cells, which set the baseline. “*”, p < 0.05 represents significance compared to control cells. F, G Invasion study of PC3 F and SNB19 G cells with RAB4A knockdown with and without concurrent expression of either wild-type RAC1 (RAC1WT) or constitutively active RAC1 (RAC1CA). Scale bar = 100 µM