Fig. 5

KLF7OE increased transcription but not translation. A Fluorescent signal after 45 min of HPG-TAMRA incorporation demonstrated an increased signal in KLF7OE cells. B Quantification of the cytoplasmic fluorescent signal showed a significantly increased translation rate in MDA-MB-231 cells and MCF7 cells. C Nuclear HPG-TAMRA intensity after 45 min incorporation. D Representative images of fluorescent signals after 5-EU incorporation labeling nascent RNA. E Cytoplasmic and nuclear intensity of 5-EU-TAMRA was unchanged upon KLF7 overexpression