Fig. 7

Cell survival effects of Paqr8 are dependent on Gi protein-mediated reductions in cAMP. A, B Intracellular cAMP measurements of Her2-dependent primary mouse tumor cells following 72 h of Her2 downregulation, with or without addition of 1μM forskolin (Fsk) for 15 min prior to collection, and with or without 12-h preincubation with 100 ng/ml pertussis toxin (PTX). C, D Viable cell counts of Her2-dependent primary tumor cells at 72 h following Her2 withdrawal, in the presence or absence of 100 ng/ml PTX. E, F Percentage of eGFP+ cells that were cleaved caspase-3 + by immunofluorescence staining of Her2-dependent primary cells cultured for 72 h after Her2 downregulation, in the presence or absence of 100 ng/ml PTX