Fig. 7From: Exosomal Wnt7a from a low metastatic subclone promotes lung metastasis of a highly metastatic subclone in the murine 4t1 breast cancerLM.4T1 tumors are highly inflammatory with increased angiogenesis. One hundred and thousand LM.4T1 or HM.4T1 cells were implanted into the 3rd mammary pad of BALB/c mice. Mice were euthanized two weeks later, and tumors were excised for analyses. A Expression of Il6, Il1b, Tgfb1, Tnfa, Vegfa, Hgf, and Col1a1 mRNA was examined by RT-qPCR. The results are presented as the mean ± SEM. Statistical significance was analyzed by two-way ANOVA with multiple comparisons. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n = 5. B Cells expressing α-SMA, CD31, or ERG were detected by immunohistochemistry. Percent positive areas for α-SMA- or CD31-positive cells were obtained using the Image J software, and the number of ERG+ was counted by eyes. The results are presented as the mean ± SEM. Statistical significance was analyzed by one-way ANOVA with multiple comparisons. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n = 4. C One hundred and thousand LM-Ctrl or LM-Wnt7a (−) cells were implanted into the 3rd mammary pad of BALB/c nude mice. Mice were euthanized two weeks later, and tumors were excised for analyses. Cells expressing α-SMA, CD31, or ERG were detected by immunohistochemistry. Percent positive areas for α-SMA- or CD31-positive cells were obtained from two sections, and the number of ERG+ was counted by the Image J software. The results are presented as the mean ± SEM. Statistical significance was analyzed by unpaired Student’s t test. *p < 0.05, n = 6. D Expression of Vegfa mRNA was examined by RT-qPCR. The results are presented as the mean ± SEM. Statistical significance was analyzed by unpaired Student’s t test. *p < 0.05, n = 9Back to article page