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Fig. 3 | Breast Cancer Research

Fig. 3

From: Exosomal Wnt7a from a low metastatic subclone promotes lung metastasis of a highly metastatic subclone in the murine 4t1 breast cancer

Fig. 3

Exosomes derived from LM.4T1 cells can promote HM.4T1 cell migration and invasion. A Transmission electron microscopic images of exosomes purified from the culture supernatant of LM.4T1 or HM.4T1 cells. The scale bar indicates 200 nm. B The presence of exosome-enriched protein markers, including CD63 and TSG101, as well as the absence of the negative exosome marker GM130 in cell lysates and exosomes of LM.4T1 and HM.4T1 cells was analyzed by western blotting. C Wound healing assay was used to investigate the migration of HM.4T1 cells co-cultured with or without exosomes from LM.4T1 cells. The results are presented as the mean ± SEM. Statistical significance was analyzed by two-way ANOVA with multiple comparisons. *p < 0.05, n = 3. D Spheroid invasion assay was used to evaluate the invasion of HM.4T1 cells co-cultured with LM.4T1-derived exosomes. The results are presented as the mean ± SEM. Statistical significance was analyzed by unpaired Student’s t test. **p < 0.01. n = 3. The scale bar indicates 100 μm

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