Fig. 5

Activated phenotype of breast CAFs. (A) Fibroblasts were embedded in collagen matrix. Surface areas of the contracted collagen disc after 8 h were analyzed with ImageJ and presented in the lower bar graph. Pictures show representative triplicate of contracted collagen discs from N = 3 independent experiments. Data are mean ± SD of N = 3. (B) Migration of breast cancer cells when co-cultured with fibroblasts. The two cell types were seeded separately in ibidi culture inserts. When cells reached confluence, the inserts were removed to allow cells to migrate w/wo GKT137831 (20 μM). After 16 h of migration, stained cells were imaged for quantification by ImageJ, as shown in (D). (C) Migration of MDA-MB231 cells in mono-culture was not significantly affected by GKT137831. Representative images of N = 3 independent experiments are shown in (B and C). Data are mean ± SD of N = 3 independent experiments. # p < 0.05 in GKT-treated samples vs DMSO