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Fig. 3 | Breast Cancer Research

Fig. 3

From: Combinatorial targeting of a chromatin complex comprising Dot1L, menin and the tyrosine kinase BAZ1B reveals a new therapeutic vulnerability of endocrine therapy-resistant breast cancer

Fig. 3

Dot1L and menin interactomes analysis in MCF-7 BC cell nuclei. A Representative western blot showing Dot1L and menin co-immunoprecipitation in MCF-7 nuclear extracts. IgG was used as negative control. B Experimental workflow for Dot1L and menin interactome identification and characterization. For each sample three independent biological replicates were performed. (C and D) Bar charts showing molecular type classification of Dot1L (left, green) and MEN1 (right, red) associated proteins. Asterisks indicate statistical significance (p* ≤ 0.05). E Venn diagram showing overlaps between Dot1L and MEN1 associated interactors. F Bar chart showing the molecular type classification of the 561 Dot1L andMEN1 common interactors. The red line indicates the number of interactors that are also fitness genes. Asterisks indicate statistical significance (p* ≤ 0.05). G Results of IPA (Ingenuity Pathway Analysis) functional analysis showing statistically significant molecular functions enriched in Dot1L and menin common interactors

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Breast Cancer Research

ISSN: 1465-542X

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