Fig. 6
BSFs that express high level of AUF1 confer chemoresistance to BC cells in a paracrine fashion. A MDA‐MB‐231 and MCF-7 cells (5 × 103 cells per well) were seeded in 96‐well plates and were incubated with TCF64-ORF-SFCM or TCF64-CTL-SFCM for 24 h and then were either sham-treated or challenged with different concentrations of cisplatin (30 and 50 μM) or docetaxel (1 and 2.5 μg/ml) for 72 h. Cell cytotoxicity was assessed using the WST1 assay and recorded as percent of survived cells relative to the untreated cells. Error bars indicate mean ± SEM (n = 3). *P ≤ 0.05. B Cells were incubated with TCF64-ORF-SFCM or TCF64-CTL-SFCM for 24 h and then were either sham-treated (DMSO) or challenged with cisplatin (30 μM) or docetaxel (2.5 μg/ml) for 72 h. Whole cell lysates were prepared and were used for immunoblotting analysis using antibodies against the indicated proteins. The numbers below the bands indicate fold changes relative to the respective control after normalization against GAPDH. C Quantifications of the immunoblotting results depicted in Fig. 5B. Error bars indicate mean ± SEM (n = 3). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001, ns: not significant