Fig. 5

BSFs that express high level of AUF1 promote tumor growth in mice. A Orthotopic breast cancer xenografts were created by co-injecting MDA-MB-231 cells with TCF64-ORF or TCF64-CTL cells into the mammary fat pads of nude mice (n = 5). Graph depicting tumor volume over time and error bars indicate mean ± SEM (n = 5). **P ≤ 0.01. B Representative photographs showing the size of the formed tumors in 2 animals from each group. C Macroscopic view of xenograft tumors upon retrieval from mice (top panel). Histogram depicting the weight of the tumors in each group (bottom panel). D Representative photographs of histologic sections subjected to hematoxylin and eosin staining and to anti‐Ki-67 antibody. Scale bar = 50 μm. E Protein extracts were prepared from the excised tumors and were used for immunoblotting analysis using specific antibodies against the indicated proteins. The numbers below the bands indicate fold changes relative to the control (T-TCF64-CTL) after normalization against GAPDH