Fig. 4

Ectopic expression of AUF1 in BSFs promotes carcinogenesis in an IL-6-dependent paracrine manner. A The indicated SFCM were applied onto human cytokine antibody array membranes (C7). B The indicated SFCM were used to assess the level of IL-6 by ELISA, and error bars represent mean ± SD (n = 3) (**P < 0.01). C Cells were treated as indicated for 24 h, and then, whole cell lysates were prepared and were used for immunoblotting analysis. The numbers below the bands represent fold change relative to the respective controls after correction against the internal control. D Cells were treated as indicated, and the migration and proliferation capacities were assessed for the indicated periods of time using the RTCA-DP xCELLigence System. Data are representative of different experiments performed in triplicate. E Cells (1000) were cultured in ultra-low attachment 96-well plates in the presence of specific stem cell medium. Left panel, representative images of mammospheres. Scale bar, 100 μm. Right panel, Graphs depicting the number of formed mammospheres. Experiments were performed in triplicate, and error bars represent means ± SD (n = 3) (*P < 0.05)