Fig. 4

Evidence that tumor-forming potential of Dusp4NULL Trp53Δ MYCAMP cells is not due to classical Dusp4 function. A Tumor growth curves of Dusp4NULL Trp53Δex7 MYCAMP cells (tumor forming) or Dusp4FLOX Trp53Δex7 MYCAMP cells (not tumor forming), with or without Cre, administered 1 week prior to injection into C57/BL6 mice. (n = 5 mice/group) B Tumor growth curves of DPM tumor cell line (derived from re-culture of dissociated Dusp4NULL Trp53Δex7 MYCAMP tumors), after transduction with GFP control, or reconstitution of human DUSP4, or phosphatase-dead DUSP4 (hDUSP4PD) (n = 5 mice/group). Inset demonstrates expression of DUSP4 by a human-specific antibody. C Nuclear and cytoplasmic fractions of DPM GFP, hDUSP4, and hDUSP4PD cell lines, serum starved and stimulated with FBS over a time course. + CON is human MDA-MB 231 cell lysate