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Fig. 5 | Breast Cancer Research

Fig. 5

From: Multiple roles for Bcl-3 in mammary gland branching, stromal collagen invasion, involution and tumor pathology

Fig. 5

Bcl-3 null mice preferentially develop a higher percentage of carcinogen-induced mammary squamous carcinomas lacking NF-κB activity. a Representative H&E-stained paraffin sections of mammary tumors from WT and bcl-3−/− mice showing (i) adenocarcinoma (microacinar pattern); (ii) predominantly squamous carcinoma; (iii) squamous (white arrow) mixed with microacinar (black arrow) adenocarcinoma; and (iv) anaplastic tumor (sarcomatoid pattern). Bar = 50µ. b Histogram depicting the percentages of total squamous/anaplastic versus microacinar adenocarcinoma histology between WT and bcl-3−/− tumors. Significantly more squamous histology was present in bcl-3−/− mammary tumors, statistical analysis; unpaired t test. c Immunofluorescence staining for smooth muscle actin (SMA) and cytokeratin-6 (CK6) in representative cryosections of microacinar versus squamous tumors. Note SMA positive cells in a ductal structure within the squamous tumor section. Bars = 100µ. d Immunoblots for Bcl-2 in the indicated lesions from bcl-3−/− and WT mice. Note the low levels of Bcl-2 in anaplastic and squamous tumors relative to microacinar tumors. e Anti-puma immunoblots of bcl-3−/− and WT in the indicated lesions (panels i and ii). Lowercase histology letters in mixed tumors indicate a less than 30% proportion of the tumor composition

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