Fig. 2
Treatment of TNBC xenograft mice with F i-CAR-T cells reduces tumor growth and increases survival. 2 × 106 MDA-MB-231-ffLuc + cells were injected in the mammary fat pad of NSG mice. 6 days later, BLI was performed to ensure uniform engraftment of tumor cells. On day 7, 4 × 106 CAR-T cells were injected intravenously with or without 250 µg/mouse anti-PD-1 mAb intraperitoneally on day 7, 10 and 14. Tumors were measured with BLI (up to week 4 post-treatment) and digital caliper once tumors were palpable. A Tumor volumes (caliper measurement) at 4 weeks post-treatment, 6 representative mice/group displayed with two-way ANOVA performed *p = 0.04, ***p = 0.0005; B Kaplan–Meier survival curve of mice (n = 5–12 per group) treated with F CAR, F CAR + mAb, F i-CAR or CD19 i-CAR; log-rank test used: **p = 0.001; C CAR-T cell numbers per mm2, total of 5 sections/tumor, 5 tumors/treatment group(n = 25); one-way ANOVA used with F CAR as comparator arm, **p < 0.01; D tumors were harvested and processed for immunohistochemistry: representative mCherry staining on tumors from each treatment group (40 ×); E tumor-bearing mice were killed 4 weeks post-CAR-T cell treatment, with TILs extracted and sorted based on mCherry expression for downstream analysis: genes involved in migration (top panel), proliferation (middle panel) and cytotoxicity (bottom panel) were selected according to Log2 fold-change and p < 0.05. Differentially expressed genes from each tumor TIL population depicted as single data points and shown as Log2 gene expression