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Fig. 5 | Breast Cancer Research

Fig. 5

From: Beta 2 adrenergic receptor and mu opioid receptor interact to potentiate the aggressiveness of human breast cancer cell by activating the glycogen synthase kinase 3 signaling

Fig. 5

GSK3 inhibition reduces cell proliferation, cell migration, and colony formation in MDA-MB-231 and MDA-MB-468 cells. GSK3 gene expression suppression was done by CRISPR technology or pharmacological blockade by SB-216763 in MDA-MB-231 and MDA-MB-468 cells. In the pharmacological blockade study, IC50 dose (30 µM) of SB216763 was used for the cell proliferation, cell migration and colony formation studies. A, D, G, J Cell proliferation was evaluated at 0, 24, 48, and 72 h using MTT assay. B, E, H, K Migration capacity of cells was evaluated using the Transwell cell migration assay. Cells were stained with crystal violet after 3 h. Images were analyzed using ImageJ. C, F, I, L Colony formation of cells was evaluated for 2 weeks. After 14 days, colonies were stained using crystal violet. Images were analyzed using ImageJ. Data are mean ± SEM values obtained from three independent experiments. **p < 0.01, ***p < 0.001

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