Fig. 8

Effect of PD-L1 and MT Oas2 on lung metastases in parous mice immediately following PD-L1 treatment. A Mammary gland (MG) weight as a percentage of body weight (BW). All tumors were excised and weighed at autopsy at the completion of PD-L1 treatment, usually 2 weeks postpartum. Error bars are standard error of the mean and p values were calculated using Student’s t test. B The number of lung metastases per section (two sections per lung) counted manually, and the area of DAB + cells stained by IHC for PyMT and quantified by QuPath, for the indicated genotypes and treatments (PD-L1 or IgG IP twice weekly), of mouse cohorts. Mice without metastases (which cannot be plotted on a log axis) are shown at the bottom of the figure marked “0.” Error bars are standard error of the mean, median value is indicated by the broken bar and p values were calculated using Mann–Whitney U test. Abbreviations are MT, mutant Oas2; WT wild type Oas2