Fig. 7

Effect of PD-L1 and MT Oas2 on lung metastases in parous mice at the ethical endpoint. A Actual tumor burden of the cohorts as a % of body weight (BW). All tumors were excised and weighed at autopsy, which was initiated when estimates of tumor volume calculated from caliper measurements reached 10% of body weight. Error bars are standard error of the mean and p values were calculated using Student’s t test. B the number of lung metastases per section, counted manually using two sections per lung 100 µm apart, and the area of DAB + cells stained by IHC for PyMT and quantified by QuPath, for the indicated genotypes and treatments (PD-L1 or IgG IP twice weekly). Mice without metastases (which cannot be plotted on a log axis) are shown at the bottom of the figure marked “0.” Error bars are standard error of the mean, median value is indicated by the broken bar and p values were calculated using Mann–Whitney U test for the two-way comparisons indicated by the extremities of the line. Abbreviations are MT, mutant Oas2; WT wild type Oas2