Fig. 4

Effect of MT Oas2 and parity on lung metastases. A Actual tumor burden of the cohorts. All tumors were excised and weighed at autopsy, which was initiated when estimates of tumor volume calculated from caliper measurements reached 10% of body weight (BW). Abbreviations are MT, mutant Oas2; WT wild type Oas2; np, nulliparous; p, parous. Error bars are standard error of the mean and p values for the two-way comparisons indicated by the extremities of the lines were calculated using Student’s t test. Each symbol is the total tumor burden for a mouse. B top panel, the number of lung metastases per section counted manually using two sections per lung, 100 µm apart, and bottom panel, the area of DAB + cells stained by IHC for PyMT and quantified by QuPath, for the indicated genotypes and parity status of the mouse cohorts (20 per group). Error bars are standard error of the mean, median value is indicated by the broken bar, and p values were calculated using Mann–Whitney U test. Sections without metastases (which cannot be plotted on a log axis) are shown at the bottom of the figure marked “0.” Each symbol is a section