Fig. 9

Knocking down or blocking PTH1R in tumors does not prevent the effects of PTHrP overexpression in vivo. A Kaplan–Meier analysis of tumor onset (top) and survival (bottom) of Tet-PTHrP;PyMT;PTH1RLox and Tet-PTHrP;PyMT;Cre;PTH1RLox mice treated with Dox from birth. B Relative expression of PTH1R in RNA from isolated tumor cells. N = 3. C, G QPCR analysis of indicated genes. Hprt1 was used as housekeeping gene. N = 3. D, H Representative immunohistochemical staining for nuclear pStat5 in tumor sections from Tet-PTHrP;PyMT;Cre;PTH1RLox mice treated with Dox and controls and from Tet-PTHrP;PyMT treated with Dox and an anti-PTH1R antibody (αPTH1R) and controls. N = 3, Scale bar 100 µm. E QPCR analysis of hPTHLH expression in RNA from whole tumors. Hprt1 was used as housekeeping gene. N = 3. F Serum calcium concentration. A minimum n = 6. Bars represent mean ± SEM, ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05