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Fig. 8 | Breast Cancer Research

Fig. 8

From: PTHrP induces STAT5 activation, secretory differentiation and accelerates mammary tumor development

Fig. 8

Overexpression of PTHrP, but not exogenously added PTHrP, activates Stat5 in tumor cells. A QPCR analysis of PTH1R expression in RNA from cultured HC11, Tet-PTHrP, PyMT and Tet-PTHrP;PyMT cells. Actb was used as housekeeping gene. A minimum of n = 3 per group. Tet-PTHrP;PyMT tumor cells were treated with Dox (2 µg/ml) or PTHrP 1–34 (100 nM) and protein lysates and RNA was prepared. B Western blot analysis of protein lysates. Left, representative immunoblots of p(Tyr694)Stat5 and total Stat5 are shown. Right, densitometric quantification of the western blots. N = 3. C QPCR analysis of the indicated milk proteins. Hprt1 was used as housekeeping gene. N = 3. D Edu incorporation in cultured Tet-PTHrP;PyMT tumor cells in response to Dox or PTHrP treatment versus control. N = 9. Bars represent mean ± SEM, ****p < 0.0001, **p < 0.01, *p < 0.05

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